Table 3.
Energetics in sham, untreated (PBS), control MSC treated (MSC), and Akt-MSC treated animals at 72 hours and 2 weeks after myocardial infarction
| Sham (n=9) |
PBS (n=7) |
MSC (n=7) |
Akt-MSC (n=9) |
|
|---|---|---|---|---|
| 72 hours | ||||
| ATP (mM) | 10.0 ± 0.7 | 9.9 ± 0.7 | 9.6 ± 0.7 | 10.0 ± 1.0 |
| PCr (mM) | 17.4 ± 1.5 | 15.1 ± 1.8* | 15.3 ± 0.6* | 16.5 ± 1.3 |
| Pi (mM) | 2.1 ± 1.1 | 1.9 ± 0.6 | 2.5 ± 0.9 | 1.7 ± 0.8 |
| |ΔG~ATP| (kJ/mo1) | 61.7 ± 0.5 | 61.1 ± 0.6 | 60.3 ± 0.4 | 61.9 ± 0.6 |
| 2 weeks | ||||
| ATP (mM) | 10.0 ± 0.4 | 10.5 ± 0.4 | 10.1 ± 0.4 | 10.1 ± 0.4 |
| PCr (mM) | 18.4 ± 0.8 | 19.2 ± 0.8 | 18.7 ± 0.5 | 19.1 ± 0.6 |
| Pi (mM) | 1.4 ± 0.5 | 1.1 ± 0.1 | 1.5 ± 0.4 | 0.9 ± 0.4 |
| |ΔG~ATP| (kJ/mo1) | 62.7 ± 0.5 | 64.1 ± 1.7 | 62.4 ± 0.7 | 65.4 ± 1.1 |
Values expressed as average ± SE
*
p<0.05 vs sham.
Abbreviations: Akt-MSC, MSCs overexpressing Akt1; |ΔG~ATP|, absolute values of free energy released from ATP hydrolysis;
MSC, mesenchymal stem cells; PBS, phosphate-buffered saline; PCr, phosphocreatine; Pi, inorganic phosphate.