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The proteasome was inhibited equally in all RCC tested. (a) Cells were treated overnight with 1, 10 or 20 nM Bortezomib and then assayed with the Proteo-glo assay (Promega). (b) Cells were treated overnight with 20 nM Bortezomib; cell lysates were used for western blotting for ubiquitinated proteins.
