Skip to main content
. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Exp Cell Res. 2010 Mar 23;316(9):1479–1488. doi: 10.1016/j.yexcr.2010.03.007

Figure 2. Assessment of AR promoter methylation in prostate cancer cell lines using methylation-specific PCR.

Figure 2

PCR was performed using unmethylated (U) and methylated (M) AR specific primers. PCR products in lanes U, but not in lanes M, indicate the presence of an unmethylated allele in DNA from the P69, M2128, M2205, PC3 and C4-2 cell lines whereas PCR products in lanes M, but not in lanes U, indicate the presence of a methylated allele in DNA from the M12 and DU145 cell lines. For control purposes we evaluated the methylation status of the BRCA1 gene. BRCA1 was unmethylated in all cell lines examined.