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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Exp Cell Res. 2010 Mar 23;316(9):1479–1488. doi: 10.1016/j.yexcr.2010.03.007

Figure 3. Assessment of AR promoter methylation using the sodium bisulfite method.

Figure 3

Genomic DNA was obtained from M12 and P69 cells, followed by sodium bisulfite treatment and direct DNA sequencing. Examples of DNA sequencing chromatograms are shown here. CpG sites are underlined. All cytosines (C) are deaminated and converted to thymines (T) in the P69 cell line, but 5-methylcytosines (shown as C) remained unaltered in the M12 cell line, meaning that the AR promoter is methylated in M12, but unmethylated in P69, cells.