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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Exp Cell Res. 2010 Mar 23;316(9):1479–1488. doi: 10.1016/j.yexcr.2010.03.007

Figure 7. Sodium bisulfite-DNA sequencing analysis of the IGF1R promoter.

Figure 7

Genomic DNA was extracted from P69 and M12 cells and modified with sodium bisulfite as described in Materials and methods. PCR was performed using a set of primers encompassing Region 2 of the proximal IGF1R promoter. Sodium bisulfite-PCR products were sequenced using the BigDye terminator method. (A) Unmodified DNA obtained from P69 cells. (B) Sodium bisulfite-modified DNA from P69 cells. (C) Sodium bisulfite-modified DNA from M12 cells.