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Primer sets and PCR conditions for AR promoter methylation analysis
| Primer | Sequence | Annealing(°C) | Product size |
|---|---|---|---|
| AR-WT F | CGCCCCCTCCGAGATCCCG | 65 | 213 bp |
| AR-WT R | CGGGCGGCGGCTTCGAAGCCG | ||
| AR-UF | TGTTTTTTTTGAGATTTTG | 43 | 213 bp |
| AR-UR | CAAACAACAACTTCAAAACCA | ||
| AR-MF | CGTTTTTTTCGAGATTTCG | 50 | 213 bp |
| AR-MR | CGAACGACGACTTCGAAACCG |
