Table 3.
Sesquiterpene product profile of purified Cop4 with (E,E)-FPP under different reaction conditions
| Conditions | Products (%)[a] | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| pH | NaCl (M) | T (°)C | Cation (10 mM) | 4s | 5s | 6s | 7s | 8s | 9s | ND[b] |
| 10.0 | 0 | 25 | Mg2+ | - | - | - | 91.1 | - | - | 8.9 |
| 5.0 | 0 | 25 | Mg2+ | - | - | - | 84.3 | - | 12.32 | 3.3 |
| 8.0 | 0 | 25 | Mg2+ | 5.7 | 2.8 | 7.4 | 29.3 | 28.2 | 10.4 | 16.1 |
| 8.0 | 0 | 4 | Mg2+ | 5.9 | 2.3 | 6.2 | 37.8 | 27.2 | 11.7 | 8.9 |
| 8.0 | 0 | 37 | Mg2+ | 7.1 | 5.3 | 12.3 | 22.3 | 22.3 | 13.3 | 17.3 |
| 8.0 | 1 | 25 | Mg2+ | 4.8 | 1.5 | 4.2 | 17.7 | 33.5 | 10.2 | 28.0 |
| 8.0 | 0 | 25 | Mn2+ | 5.5 | - | 2.3 | 48.7 | 20.2 | 7.1 | 16.2 |
| 8.0 | 0 | 25 | K+ | 10.7 | 3.7 | 10.3 | 19.4 | 34.2 | 15 | 11.3 |
Structures corresponding to compound numbers shown are found in Scheme 1.
[a]
Percentages are averages calculated from product profiles of three independent in vitro reactions. The error was less than 5% in all cases.
[b]
ND: Sum of minor products that could not be identified.