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Expression of DappuE75 in S2 cells transfected with 0.25 μg of pMT-E75 vector (lane 1) or and DappuHR3 in cells transfected with 2.0 μg of pMT-HR3 vector (lane 2). All cells were also transfected with 0.5 μg pMT-lacZ (β-Gal). Proteins were detected with α-V5 tag antibodies.