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. Author manuscript; available in PMC: 2010 May 19.
Published in final edited form as: Biochemistry. 2008 Jan 8;47(5):1414–1424. doi: 10.1021/bi701955e

Figure 10.

Figure 10

Evidence for RET between Trp101 and bound ANS in the C101W–ANS complex. (A) Key: dashed line, corrected excitation spectrum (emission at 500 nm) of the W17Y (5.0 μM)–ANS (2.5μM) complex; solid line, corrected excitation spectrum (emission at 500 nm) of the C101W (5.0 μM)–ANS (2.5 μM) complex. (B) Key: dashed line, excitation spectrum (emission at 337 nm) of C101W (5.0 μM) without ANS; solid line, difference excitation spectrum at an emission of 500 nm [spectrum of C101W–ANS – 0.63(spectrum of the W17Y–ANS complex shown in (A))].