Docking solution for interaction of ANS with TL. The backbone of TL (PDB 1XKI) is shown in cyan in a ribbon representation. Gray, blue, red, and yellow sticks represent carbon, nitrogen, oxygen, and sulfur atoms, respectively. The position of ANS (energy level –11.0 kcal/mol) is that in best agreement with the experimental data. Distances, indicated with green dotted arrows, between the sulfonate group of ANS and the side chains of Glu34, His84, and Lys114 are 2.5, 2.4, and 3.0 Å, respectively. The image was generated by DeepView/Swiss-PdbViewer v.3.7 (GlaxoSmithKline R&D).