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Quenching the steady-state fluorescence of single Trp mutants by ANS. The fluorescence intensities were measured at the wavelengths of the respective emission maxima. Emission λ values in the experiments with L105W, C101W, M39W, and E34W were 348, 337, 332, and 344 nm, respectively. The excitation λ was 295 nm. The solid curves are the best fit for one binding site model.
