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. Author manuscript; available in PMC: 2011 May 15.
Published in final edited form as: Cancer Res. 2010 Apr 27;70(10):4005–4014. doi: 10.1158/0008-5472.CAN-09-3912

Figure 2. IKKβ regulates critical target genes in ovarian cancer.

Figure 2

IKKβ target genes in ovarian cancer: A – CAOV3 cells were treated with the IKKβ inhibitor ML120b for the indicated times and gene expression changes were assessed using Affymetrix U133plus2.0 microarrays and depicted according to the color scale shown. B – IKKβ was depleted in CAOV3 cells by retroviral transduction of targeted shRNA. Regulated genes were identified by class comparison in six replicates compared to control shRNA. C – IKKβ target gene regulation was confirmed with quantitative RT-PCR after shRNA-mediated depletion of IKKβ. D – Specificity of shRNA target was confirmed by western blot demonstrating depleted protein expression of IKKβ, but not IKKα or IKKε. Functionally, IKKβ depletion resulted in decreased phosphorylation of IκBα and lower level of IKKβ target Claudin1.