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. Author manuscript; available in PMC: 2011 May 1.
Published in final edited form as: Virus Res. 2010 Feb 4;149(2):190–196. doi: 10.1016/j.virusres.2010.01.017

Fig. 1. Nucleotide polymorphisms of the four BKV subtypes alter the virus growth efficiency in Vero cells.

Fig. 1

Vero cells were infected with BKV strains (BKV I, II, III, and IV) containing the nucleotide polymorphisms of the four BKV subtypes. During the infection course, aliquots of culture medium and cell pellets were collected and viral replication was assessed by Q-PCR. A. the graph shows the number of genomic copies of BKV I, II, III, and IV detected in the culture medium samples collected at days 5, 11, 17, 31, 47, 59 P.I. (data were expressed as viral copies per ml of culture medium). Day 24 P.I. is not indicated in the graph since aliquots of culture medium were not collected at this time point. B. the graph shows the number of copies of BKV I, II, III, and IV detected in aliquots of Vero cells collected at days 5, 11, 17, 24, 31, and 59 P.I. (data were expressed as viral copies per μg of DNA extracted from cell pellets). Day 47 P.I. is not indicated in the graph since aliquots of Vero cells were not collected at this time point. Both graphs indicate that BKV I, II, and IV share similar replication patterns. On the contrary, the polymorphisms of subtype III negatively affect the in vitro propagation of the virus, since viral titres of BKV III detected in both culture medium and cell pellets progressively decreased during the course of infection.