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. Author manuscript; available in PMC: 2011 May 1.

Published in final edited form as: Virus Res. 2010 Feb 4;149(2):190–196. doi: 10.1016/j.virusres.2010.01.017

Fig. 2 — Vero cells were infected with BKV I, II, IV and BKV strains (BKV I Gln 68, II Gln 68, and IV Gln 68) whose VP1 contained the amino acid polymorphisms of subtypes I, II, and IV, with the exception of position 68, where a Leu was substituted by a Gln, as in subtype III. During the infection course, aliquots of culture medium and cell pellets were collected and viral replication was assessed by Q-PCR. A, B, C. the graphs compare the number of genomic copies of BKV I and I Gln 68, BKV II and II Gln 68, BKV IV and BKV IV Gln 68, respectively, detected in the culture medium samples collected at days 5, 12, 18, 25, 32, 39, 46 P.I. (data were expressed as viral copies per ml of culture medium). D, E, F. the graphs compare the number of genomic copies of BKV I and I Gln 68, BKV II and II Gln 68, BKV IV and BKV IV Gln 68, respectively, detected in aliquots of Vero cells collected at days 5, 12, 18, 25, 32, 39, 46 P.I. (data were expressed as viral copies per μg of DNA extracted from cell pellets). On the whole, all the viral strains were detected with progressively higher viral titres during the infection course, thus indicating that the presence of a Gln instead of a Leu at position 68 of VP1 does not affect the replication efficiency of subtypes I, II, and IV.

Fig. 2 — Vero cells were infected with BKV I, II, IV and BKV strains (BKV I Gln 68, II Gln 68, and IV Gln 68) whose VP1 contained the amino acid polymorphisms of subtypes I, II, and IV, with the exception of position 68, where a Leu was substituted by a Gln, as in subtype III. During the infection course, aliquots of culture medium and cell pellets were collected and viral replication was assessed by Q-PCR. A, B, C. the graphs compare the number of genomic copies of BKV I and I Gln 68, BKV II and II Gln 68, BKV IV and BKV IV Gln 68, respectively, detected in the culture medium samples collected at days 5, 12, 18, 25, 32, 39, 46 P.I. (data were expressed as viral copies per ml of culture medium). D, E, F. the graphs compare the number of genomic copies of BKV I and I Gln 68, BKV II and II Gln 68, BKV IV and BKV IV Gln 68, respectively, detected in aliquots of Vero cells collected at days 5, 12, 18, 25, 32, 39, 46 P.I. (data were expressed as viral copies per μg of DNA extracted from cell pellets). On the whole, all the viral strains were detected with progressively higher viral titres during the infection course, thus indicating that the presence of a Gln instead of a Leu at position 68 of VP1 does not affect the replication efficiency of subtypes I, II, and IV.