Table 2.
Name | Sequence |
---|---|
BKV I Gln | 5′-GGGGGATCCaAGATGAAAACCTTAGGGGCTTTAGTCAGbAAGCTAAG-3′ |
BKV II Gln | 5′-GGGGGATCCaAGATGATAACCTTAGGGGCTATAGTCAGbAAGCTAAC-3′ |
BKV IV Gln | 5′-GGGGGATCCaAGATAATGACCTTAGGGGCTATAGTCAGbAGACTAAC-3′ |
BamHI restriction site;
nucleotide mismatches (positions 1766–1767) between the forward primers and the template DNA (pucDun, puc II, and puc IV, respectively), which bears nucleotides TA at that genomic position.