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. 2010 May 4;123(11):1873–1883. doi: 10.1242/jcs.061242

Fig. 3.

Fig. 3.

Increased chemical sulfation results in a moderate increase in satellite-cell proliferation whereas chemical de-sulfation results in a slight decrease. Satellite cells on single fibres grown in culture medium containing 8 mM sodium sulfate (A-F) or 8 mM sodium chlorate (G-J), or 8 mM sodium chlorate+100 ng/ml Sulf1A antibody, stained after 24, 48 and 72 hours for Pax7 (column1 A-C) or for MyoD (column 1 D-I) or for sulfation with antibody 10E4 (J, K) and Sulf1A (column 2) using a double immunofluorescence procedure. The superimposed images of Pax7 and Sulf1A or MyoD and Sulf1A or 10E4 and Sulf1A staining are shown in column 3 with additional DAPI staining in column 4. CLO3, chlorate; SO4, sulfate. Anti-Sulf1A antibody was used at a concentration of 100 ng/ml.