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Microtitre plate of seedlings inoculated with Agrobacterium, treated by sonication, needle wounding or mixing by vortex with carborundum and stained for β-glucuronidase (GUS)Plus activity. (a) Control (sonicated) switchgrass seedlings; (b) sonicated seedlings that were needle inoculated; (c) sonicated seedlings that were mixed by vortex with carborundum. All seedlings were assayed for GUSPlus activity.
