Table 1.

Comparison of GUSPlus expression in switchgrass seedlings induced by four Agrobacterium strains, at different acetosyringone concentrations.

Agrobacterium strains	Acetosyringone, μM				GUS-positive seedlings
	0	50	100	200	Mean ± SD*	Total, n†	%
AGL1	2	6	14	14	9.0 ± 3.0a	36	6.0
EHA105	2	4	7	7	5.0 ± 1.2b	22	3.7
GV3101	2	2	2	1	1.5 ± 0.3c	7	1.2
C58	1	1	0	0	0.5 ± 0.3c	2	0.1

AS = acetosyringone; GUS = β-glucuronidase.

*Means followed by the same letter (a, b, c) were not significantly different at the 5% level using The Tukey multiple comparison test.

† Three replicates of 50 seedlings tested with each strain at each acetosyringone concentration.