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Flattening of D. discoideum under agar and oil overlayer. cAR1-GFP labeled cells below an agar (A, C) and oil (B, D) overlayer. (A) and (B) show confocal x-y scans. (C) and (D) show the corresponding z-stacks of those cells. The blue lines in (A, B) show the y-values of sections (C, D), and the blue lines in (C, D) show the z-value of images (A, B). Scale bar: 10 μm.
