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. 2010 Apr 19;3:9. doi: 10.1186/1757-5036-3-9

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Copyright ©2010 Beta et al

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Characterization of the single layer microfluidic device. (A) The channel was filled with 100 μM fluorescein, and the intensity in the middle of the device was recorded. Between t = 85 s (line 1) and t = 685 s (line 2), the syringe pump withdrew 250 μl/h of the fluorescein through the device, leading to a drop in the channel height. This flow rate was used to flatten cAR1-GFP cells. Confocal x-y scans are shown for a cAR1-GFP cell (B) before and (C) during flattening. (D, E) show the corresponding x-z sections. The blue lines in (B, C) show the y-values for the sections (D, E), and the blue lines in (D, E) show the z-value of images (B, C). Scale bar: 10 μm.