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. 2010 May 19;5(5):e10723. doi: 10.1371/journal.pone.0010723

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Tobler, Nijhout.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A ligation was placed between the second and third thoracic segment on the first day of the wandering phase (day 6). The ligation prevented the forewing from receiving most factors coming from the abdominal region, and also prevented the hindwing from receiving brain factors. The relationship between fore- and hindwing sizes for controls fall on a common regression. In ligated larvae (open triangles) the forewing disks grew little in 24 hours, and the hindwings grew about half as much as controls (black circles) The regression lines for control and ligated larvae for fore- vs hindwing disk sizes are statistically different (p<0.001).