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. 2010 Apr 21;8:40. doi: 10.1186/1479-5876-8-40

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Copyright ©2010 Radaelli et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Prime-boost protocols for rabbit immunisation. Animals were immunised i.d. every month and bled before each inoculation. Four immunisation protocols were applied. In Protocol 1, the animals were immunised with the FP_E6recombinant (10⁸PFU/animal), in Protocol 2 with the FP_E7recombinant (10⁸PFU/animal), in Protocol 3 with the FP_E6+ FP_E7recombinants (10⁸PFU/recombinant/animal), in Protocol 4 with FPwt (10⁸PFU/animal). In Protocols 2 and 3 the animals were also boosted with the recombinant E7 protein (100 μg/boost). Rabbits # 50, 62 and 83 died for natural reasons before the fifth priming. T1-T5, immunisation times for priming; P1-P4, immunisation times for protein boost.