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. 2010 Apr 21;8:40. doi: 10.1186/1479-5876-8-40

Figure 2.

Figure 2

Analysis of the anti-E6 and anti-E7 humoral responses. Anti-E6 and anti-E7 antibody titres were determined by ELISA, after plating the E6 (Panel A) or E7 (Panel B) antigens. Heat-inactivated immuno-adsorbed sera were diluted 1:25 or 1:250 for protein-coated plates, and 1:4000 for plates coated with CaSki lysates. The reactions were revealed with goat anti-rabbit HRP-conjugated sera (1:1000) and TMB substrate. The rabbit pre-immune serum from each animal was used as a negative control. Protein boosting with pE7 increased the anti-E7 antibody titres after priming with either FPE7 (Protocol 2) or with FPE6 + FPE7 (Protocol 3/E7). When CaSki lysates were used, the level of detected antibodies was much higher than after plating the purified proteins with a significant increase of E6 (Protocol 3/E6) and E7 antibodies after priming (Protocol 3/E7) and boosting (Protocol 3/E7). Statistical significances using the ANOVA parametric test are shown: (*) p < 0.05; (**) p < 0.01; (***) p < 0.001.