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. 2010 Apr 21;8:40. doi: 10.1186/1479-5876-8-40

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Copyright ©2010 Radaelli et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Th1/Th2 cytokine determination by the QuantiGene 2.0 Reagent system. The RNAs of the different PBMC samples from all of the bleeding times were used in duplicate to determine the levels of expression of the rabbit IFN-γ (Panel A) and IL-4 (Panel B) transcripts. In all of the animals, the Th2 response was generally higher than Th1 and, in particular, in FP_E6-immunised animals of Protocol 1 IL-4 production was significantly higher than IFN-γ. IFN-γ production was significantly higher in Protocol 3 than in Protocol 2 both during priming and after the E7 protein boosting. IFN-γ and IL-4 levels are expressed as fold-differences vs. baseline, obtained from non-stimulated pre-immune PBMCs, and normalized against β-actin expression. Statistical significances using the Student t-test are shown: (*) p < 0.05; (**) p < 0.01; (***) p < 0.001.