Table 1.

Effect of PhaR based protein purification method for purifying EGFP, MBP and LacZ, respectively

	Yield of purified protein		LacZ activity (Units/mg a)
Target proteins	mg/g nanoparticles	mg/g wet weight of bacteria	Pre-induced whole cell lysate	Induced whole cell lysate	Purified LacZ
EGFP	9.14 ± 1.2	4.39 ± 0.5
MBP	8.17 ± 1.06	4.01 ± 0.3
LacZ	6.55 ± 0.9	3.16 ± 0.3	7.22 ± 0.9	167 ± 7.5	486.77 ± 15

^a One unit of β-galactosidase activity hydrolyzes 1.0 μmol ONPG to o-nitrophenol and D-galactose per minute at 37°C.