Figure 5. Ability of the WT and recombinant viruses to rescue VSV from the anti-viral action of IFN-α.

HeLa cells were mock-infected or infected with the indicated viruses. At 6 h p.i., the media were replaced with fresh media containing IFN-α (1,000 IU/ml) or no IFN-α. After an additional 6-h incubation, cells were superinfected with rVSV-GFP. After further incubation for 6 h, GFP expression derived from rVSV-GFP replication was observed under a fluorescent microscope. (B) Cell lysates were analyzed by Western blotting to detect expression of rVSV-GFP-derived GFP, SeV P, and C proteins. (C) The amounts of GFP in the cell lysates of Fig. 5B were quantitated and shown as a bar graph. The value of the mock-infected, IFN-α-non-treated sample was set to 1. Bars represent the average for three independent experiments.