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. 2010 Apr 15;9:77. doi: 10.1186/1476-4598-9-77

Figure 2.

Figure 2

Over-expression of survivin in various 17-AAG/geldanamycin-treated human cancer cell lines. (A) Human HONE-1 cancer cells were treated with various concentrations of 17-AAG/geldanamycin for 24 hours. Cell lysate was prepared after the treatment and various proteins were resolved by SDS-PAGE. The expression of Akt and survivin was revealed by Western blot analysis. Actin was used as an internal control. Relative ratio of expression was shown. (B) Human HT-29 cancer cells were treated with various concentrations of 17-AAG/geldanamycin for 24 hours. Cell lysate was prepared after the treatment and various proteins were resolved by SDS-PAGE. The expression of Akt and survivin was revealed by Western blot analysis. Actin was used as an internal control. Relative ratio of expression was shown.