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. 2010 Apr 15;9:77. doi: 10.1186/1476-4598-9-77

Figure 7.

Figure 7

Inhibition of Hsp90 with 17-AAG reduced proteasome activity in HONE-1 and HT-29 cells. (A) Cells were treated with various concentrations of 17-AAG for 24 h. Cells were washed with PBS and lysed with TNESV buffer without protease inhibitor. Cell lysate were analyzed for proteasome activity using synthetic fluorogenic peptide succinyl-Leu-Leu-Val-Tyr-7-amino-4-methylcoumarin. Statistical significance (p < 0.05) between experimental samples and the DMSO control is denoted by "*". (B) Inhibition of 26S proteasome further increased the amount of survivin protein in 17-AAG treated HONE-1 and HT-29 cells. Cells were treated with DMSO and various concentrations (100 nM, 250 nM, 500 nM) of 17-AAG with/without MG-132 for 24 hours. The expression of survivin was revealed by Western blot analysis. Actin was used as an internal control.