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Complementation of E. coli mutants deficient in xylose isomerase or xylulokinase by C. acetobutylicum genes. The empty vector was expressed in the same strain as a negative control. The ability of xylose utilization was tested using MacConkey agar supplemented with 10 g l-1 xylose. The colonies of cells with the ability in xylose utilization showed red color, whereas the colonies of cells unable to utilize xylose keep yellow.
