Table 6.
Annotation of genes in the B. fragilis 638R Bfgi2 insertion.
| ORF | Protein Length |
Putative function | % Id/Sima | Organism (Bacteriophage)b | Accession no.c |
|---|---|---|---|---|---|
| 1 | 446 | Integrase | 47/63 (436) | Bacteroides uniformis | AAF74437.1 |
| 2 | 751 | Polysialic acid transport protein, KpsD | 72/84 (676) | B. fragilis YCH46 | BAD48680.1 |
| 3 | 163 | Hypothetical protein | 37/49 (156) | B. fragilis YCH46 | BAD49193.1 |
| 4 | 172 | N-acetylmuramyl-L-alanine amidase | 60/75 (150) | B. thetaiotaomicron | AA077433.1 |
| 5 | 151 | Holin | 25/54 (99) | B. subtillus (phi-105) | NP_690778.1 |
| 6 | 1215 | Phage related protein, tail component | 26/49 (173) | Actinobacillus pleuropneumonia | ZP_00134779.1 |
| 7 | 697 | Hypothetical protein | 21/40 (300) | Flavobacterium (11b) | YP_112519.1 |
| 8 | 1034 | Tail tape measure protein | 31/50 (119) | Burkholderia cepacia (BcepNazgul) | NP_918983.1 |
| 9 | 195 | Hypothetical protein | 32/54 (150) | B. fragilis YCH46 | BAD49201.1 |
| 10 | 126 | Hypothetical protein | 29/52 (86) | B. fragilis YCH46 | BAD49202.1 |
| 11 | 425 | Phage major capsid | 32/50 (252) | Vibrio phage VP882 | AAS38503.2 |
| 12 | 204 | Prohead protease | 42/59 (157) | Lactobacillus casei (A2) | CAD43895.1 |
| 13 | 450 | Phage portal protein | 34/52 (365) | Pseudomonas (D3) | AAD38955.1 |
| 14 | 543 | Terminase (Large subunit) | 38/58 (493) | Streptococcus agalactiae (λSa04) | ABA45667.1 |
| 15 | 145 | Terminase (Small subunit) | 26/43 (122) | Lactococcus lactis (Bil309) | NP_076733.1 |
| 16 | 139 | Hypothetical protein | 28/59 (171) | Clostridium difficile 630 | CAJ67750.1 |
| 17 | 104 | HNH Endonuclease | 41/59 (74) | Geobacillus (GBSVI) | ABC61271.1 |
| 18 | 142 | Hypothetical protein | 98/100 (136) | B. fragilis YCH46 | BAD49213.1 |
| 19 | 104 | Hypothetical protein | 97/100 (93) | B. fragilis YCH46 | BAD49214.1 |
| 20 | 320 | Hypothetical protein | 99/100 (294) | B. fragilis YCH46 | BAD49215.1 |
| 21 | 113 | Hypothetical protein | 99/99 (109) | B. fragilis YCH46 | BAD49216.1 |
| 22 | 428 | Ctn003 | 39/53 (420) | B. fragilis YCH46 | AAS83476.1 |
| 23 | 175 | Ctn002 | 35/48 (134) | B. fragilis YCH46 | AA583475.1 |
| 24 25 |
253 137 |
Putative DNA Methylase | 100/100 (253) | Lactococcus lactis (Tuc2009) | NP_108695.1 |
| 26 | 124 | Hypothetical protein | 88/88 (116) | B. fragilis YCH46 | BAD49220.1 |
| 27 | 150 | NinG recombination protein | 98/98 (125) |
A. actinomycetemcomitans (AaPhi23) bacteriophage bb bacteriophage |
NP_852744.1 |
| 28 | 126 | Hypothetical protein | 93/94 (116) | B. fragilis YCH46 | YP_099756.1 |
| 29 | 149 | DNA Topoisomerase I | 32/51 (82) | Pediococcus pentosaceus ATCC25745 | YP_80446.1 |
| 30 | 106 | Excisionase | 42/61 (52) | Colwellia psychrerythraea 34H | YP_268668.1 |
| 31 | 198 | Hypothetical protein | 66/74 (110) | B. fragilis YCH46 | BAD49224.1 |
| 32 | 137 | Peptidase S24 | 29/50 (81) | Flavobacterium johnsoniae | EASS8507.1 |
| 33 | 121 | Hypothetical protein | 35/52 (120) | Pelobacter carbinolicus | YP_358455.1 |
| 34 | 431 | C10 protease | 28/45 (375) | B. thetaiotaomicron | NP_811364.1 |
a Percentage identity/similarity, the number in parenthesis is the number of amino acids used in the calculations.
b The organism, with associated bacteriophage in parenthesis where applicable.
cAccession number of the highest scoring BLAST hit with an annotated function.