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. 2010 Apr 23;17(1):30. doi: 10.1186/1423-0127-17-30

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Copyright ©2010 Yao et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Dose- and time-dependent changes in expression of selected genes upon exposure to 17-AAG. After the ARPE-19 cells were treated with either 3 μM 17-AAG for 16, 24 or 48 h, or 2, 5, or 10 μM 17-AAG for 24 h, total RNA from each sample was extracted and reverse transcribed into cDNA. Changes in the expression of proteins were verified by real-time quantitative PCR. The dose (A) and time (B) -dependent changes were both evaluated as mean ± SD. The changes in selected genes are consistent with the results of proteomics. Another three unidentified proteins, Hsp70, Hsp90α and Hsp90β, were all upregulated by 17-AAG treatment, but their upregulation declined in a time and dose-dependent manner.