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Dye migration in denaturing polyacrylamide gels.
| Acrylamide solution (%) |
Bromophenol blue (BB) (nt) |
Xylene cyanol (XC) (nt) |
|---|---|---|
| 5 | 35 | 130 |
| 6 | 26 | 106 |
| 8 | 19 | 75 |
| 10 | 12 | 55 |
| 20 | 8 | 28 |
The dyes (BB and XC) co-migrate with RNA or DNA fragments of a specific length, as shown above (number of nucleotides). Importantly, this correlation varies with the percentage of the gel.
