Skip to main content
. Author manuscript; available in PMC: 2010 May 19.
Published in final edited form as: Nat Protoc. 2008;3(5):811–823. doi: 10.1038/nprot.2008.45
Component Amount per reaction Final
Plasmid DNA Variable 250 μg
10× Restriction enzyme buffer 50 μl 1×
100× BSA (if necessary) 5 μl 1×
Restriction enzyme (20 U μl−1) 20 μl 400 U
Reaction volume 500 μl