Figure 1.
BI-1 mRNA analysis by quantitative absolute real-time PCR using SYBR Green I: in control specimens (Control), liver samples from patients with chronic hepatitis (CH), cirrhosis (CIRR), cirrhotic tissues surrounding hepatocellular carcinoma (PHCC) and HCC (1A); in HCV-infected liver tissues (1B); in CH HCV-related tissues according to genotypes (1C). The unknown BI-1 and β-actin mRNA amounts in the samples were extrapolated by the respective standard curves performed using serial diluition (1:10) from 108 to 102 copies/μl of a reference sample. Form each sample the normalized amount of the BI-1 transcripts was obtained by the ratio of BI-1 to β-actin, used as housekeeping gene. Data are expressed as mean values ± SD.