Table 2.
Transcripts altered in a Δlpp mutant of Y. pestis CO92 upon temperature shift from 26°C to 37°C, compared to the wild-type strain, based on microarray analyses.
| Yer ID | Gene Name | Gene Symbol | Function | Δlpp/WT |
|---|---|---|---|---|
| FC | ||||
|
| ||||
| Cell envelope | ||||
|
| ||||
| YPO2394 | major outer membrane lipoprotein | mlpA | lpp gene - cell envelope | −1138 |
| YPO1527 | putative membrane protein | — | Cell envelope | −1.7 |
| YPO2417 | putative membrane protein | — | Cell envelope | 2.0 |
|
| ||||
| Metabolism | ||||
|
| ||||
| YPO2404 | conserved hypothetical protein | — | Iron-sulfur cluster assembly scaffold protein | −2.2 |
| YPO0408 | putative aldolase | — | Energy metabolism | −115.9 |
| y1235 | putative ATP-binding protein of ABC transport system | — | Inorganic ion transport and metabolism | −1.7 |
| y0015 | malate synthase A | aceB | TCA cycle | −2.1 |
| y0176 | succinate-semialdehyde dehydrogenase | gabD | Gamma-aminobutyrate metabolism | 1.5 |
|
| ||||
| Protein secretion and trafficking | ||||
|
| ||||
| YPO0502 | similar to hemolysin-coregulated protein (Hcp) | — | Possible type VI secretion system effector | −52.8 |
| YPO3275 | Clp ATPase | htpM | Type VI secretion system clpB chaperone | −2.0 |
| YPCD1.62 | putative type III secretion regulatory protein | lcrQ/yscM | Blocks yop transcription | −5.3 |
| YPO2597 | sec-independent protein translocase protein | tatE | Protein and peptide secretion and trafficking | −1.7 |
|
| ||||
| Stressor response | ||||
|
| ||||
| YPO3643 | major cold shock protein Cspa2 | cspa2 | Stress response | 3.0 |
| y0224 | cold shock-like protein | cspI | Stress response | 3.8 |
| y0137 | serine endoprotease | degQ | Protease/chaperon activated in response to stress | −1.9 |
| YPO4085 | heat shock protein | htpN/hslT | Protein folding and stabilization, stress response | −3.5 |
| YPO3382 | global stress requirement protein GsrA | htrA/degP | Protease/chaperon activated in response to stress | −2.9 |
| YPO2897 | DNA-binding transcriptional regulator IscR | iscR | Iron-sulfur cluster assembly, stress response | −2.0 |
| YPO0238 | mechanosensitive ion channel | mscL | Turgor regulator, activated in response to stress | −2.5 |
| YPO2896 | putative aminotransferase | nifS/iscS | Iron-sulfur cluster assembly, stress response | −1.9 |
| YPO3969 | universal stress protein B | uspB | Stress response | −1.9 |
|
| ||||
| Toxin production and resistance | ||||
|
| ||||
| YPO0337 | similar to subtilase cytotoxin, subunit B | — | Putative toxin and probable virulence factor | −2.0 |
| YPO0431 | osmotically inducible protein Y | b4376 | Toxin production and resistance | −55.7 |
| YPO2333 | quaternary ammonium compound-resistance protein | qacE | Toxin production and resistance | −4.1 |
|
| ||||
| Virulence factors | ||||
|
| ||||
| YPO2145 | similar to the Bacillus subtilis stage V sporulation protein R | — | Involved in spore cortex formation in B. subtilis | −3.2 |
| YPPCP1.05c | pesticin | pst | Bacteriocin that induces the formation of spheroplasts | −1.7 |
| YPO3138 | modulating protein YmoA (histone-like protein) | ymoA | Protein modification and repair, invasion | −2.2 |
|
| ||||
| Unknown functions | ||||
|
| ||||
| various genes (y1333, 1850, 3268, YPO0102, 2307, 3137, 3518, 3707, and 4064) | * | |||
Functions were obtained from the CMR online database (http://cmr.jcvi.org) and from the literature. FC = fold-change, which was calculated as the ratio between the hybridization signal for that gene in the Y. pestis Δlpp mutant and WT bacteria (Δlpp/WT). Expression differences were deemed as statistically significant if the fold-change was ≥1.5 and P value ≤.05. A negative sign (“−”) before the FC indicates down-regulation in the Y. pestis Δlpp mutant, relative to the WT strain. *FCs for genes with unknown functions (grouped together in the last line) ranged from −1.8 to −140.6.