Fig. 2.

Gemcitabine prevents viral replication in PT45 and Suit2 pancreatic cell lines and in normal cells. A, replication in PT45 and (B) Suit 2 cells was analyzed after 48 h (grey) and 72 h (black) of treatment with 10 nmol/L gemcitabine, and (C) NHBE cells after 48 h and 72 h of treatment with viruses alone (black or grey) and in combination with gemcitabine at 10 nmol/L (striped) and 10 μmol/L (crossed).Virus and drug were added simultaneously and both cells and media were analyzed byTCID₅₀ assays. The data are from a representative of three experiments. The * indicates that replication levels were below the detection limit of the assay, < 1pfu/cell. D, QPCR of viral DNA in PT45 cells 24 and 48 h after infection.Viral DNA copies were determined after treatment with 10 nmol/L (striped) and 10 μmol/L (crossed) gemcitabine or with the Ad5ΔE1B19K virus alone (black). Gene amplification was normalized to input virus (3 h after infection; n = 3).