Figure 2. HIV-1 does not induce type 1 IFN responses in MDM.
Stimulation of M-CSF differentiated MDM with poly I:C (10 μg/mL) or Infuenza A (MOI 3) induces siginificantly increased gene expression of IFNβ (A) and IP10 (B) quantified by qPCR (p<0.01, Wicoxon sign-ranked test of paired samples). Neither macrophage tropic HIV-1 strains induced detectable expression of either of these genes. Comparable observations are evident in alternatively differentiated MDM after 4 h stimulation (C-D) and after 24 h stimulation (E-F). Individual data paints are shown for each experiment.