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. Author manuscript; available in PMC: 2010 May 20.
Published in final edited form as: AIDS. 2009 Nov 13;23(17):2255–2263. doi: 10.1097/QAD.0b013e328331a4ce

Figure 5. MDM lack constitutive or IFN-inducible expression of a functional PRR for HIV-1.

Figure 5

IFNβ (A) and IP10 (B) gene expression levels, measured by qPCR and normalized to GAPDH, are shown for M-CSF differentiated MDM at 4 h and 24 h and at 4 h for IFNβ-primed (2ng/mL 24 h before infection) and unprimed control MDM (D) after infection or stimulation with the panel of reagents indicated. In contrast to the significant response to Infuenza (p<0.01, Wicoxon sign-ranked test of paired samples), no significant IFNβ or IP10 response was evident to any of the lentiviruses. IFNβ priming consistently enhanced the response to poly I:C stimulation but not HIV-1 or VSV-G pseudotyped lentiviral vector. Values for at least 3 separate experiments shown. (C) IFNβ-induced upregulation of IP10 and selected viral PRRs is shown in an expression matrix, derived from microarray transcriptional profiling of 4 h IFNβ-stimulated and control M-CSF differentiated MDM. * denotes genes that are significantly upregulated by IFNβ (p<0.05, t-test).