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. Author manuscript; available in PMC: 2010 May 20.
Published in final edited form as: Autophagy. 2009 Nov 2;5(8):1118–1130. doi: 10.4161/auto.5.8.9991

Figure 5.

Figure 5

Differentiating atg5-/- MEFs exhibited higher rates of apoptosis. (A) Primary atg5+/+ or atg5-/- MEFs were induced for adipogenesis. The progress of differentiation and apoptotic cell death was analyzed with Bodipy 493/503 staining (green), DAPI staining (blue) and TUNEL assay (red), respectively. The pictures showed cells at Day 6 post-differentiation induction. Representative low (with a scale bar of 50 μm) and higher (with a scale bar of 10 μm) magnification pictures are shown. (B) Quantification of the TUNEL positive cells as a percentage of Bodipy 493/503 positive cells at the indicated time points. Total number of Bodipy 493/503 positive cells and total number of both TUNEL positive cells and Bodipy 493/503 positive cells in randomly selected regions were counted and the percentage was calculated. The data are representative results from three independent experiments. **p < 0.01; Student t-test.