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. Author manuscript; available in PMC: 2010 May 20.
Published in final edited form as: Autophagy. 2009 Nov 2;5(8):1118–1130. doi: 10.4161/auto.5.8.9991

Figure 6.

Figure 6

The atg5-/- mice had fewer subcutaneous fat cells. atg5-/- embryos (E18.5) and neonatal pups (within 12 hours after birth) and their wild-type littermates were obtained and the transverse sections at the level of scapulae were analyzed by immunofluorescence microscopy with primary antibody against perilipin A and FITC-conjugated secondary antibody. (A) Subcutaneous regions of embryos showing perilipin A positive adipocytes. (B) Subcutaneous regions of neonatal pups showing perilipin A positive adipocytes. (C) quantification of (A). Total number of perilipin A positive cells in subcutaneous regions of three adjacent scapulae sections were counted and averaged. (D) quantification of (B). Total number of perilipin A positive cells in subcutaneous regions of three adjacent scapulae sections were counted and averaged. The data are representative results from three independent pairs of pups born to two independent pairs of parents and three pairs of embryos born to three pairs of parents. **p < 0.01; ***p < 0.001. Student t-test.