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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Cancer Invest. 2009 Jul;27(6):613–623. doi: 10.1080/07357900802653464

Figure 2.

Figure 2

Comparison of CA mRNA expression in human breast cancer lines. RNA was isolated from subconfluent and confluent MDA-MB-231, T47D, and MCF10A cells exposed or not to DFO (100 µM) or to hypoxic conditions (1% oxygen) for 16 hr. RT-PCR was performed as described. C = control; D = DFO; H = hypoxia; m = markers; St = RT-PCR of RNA isolated from select human tissue. The standard RNA for CAIV came from heart; the standard for CAIX came from stomach; the standard for CAXII came from kidney; the standard for CAXIV, CAII, and GAPDH came from liver. GAPDH is used as a control for the RT-PCR reaction. These data represent at least two independent sets of cells.