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. 2003 Jan;9(1):116–119. doi: 10.3201/eid0901.020149

Table. Results of serologic testing, polymerase chain reaction analyses, and hamster injection for specimens from the case-patient with babesiosis and the implicated blood donora.

Date of specimen Timing of specimen Test Result of
testing
Case-patient



September 21, 2000
Pretransplant
Babesia microti IFA
<1:8b
November 14, 2000
Posttransplant
B. microti IFA
1:1024
November 14, 2000
Posttransplant
B. microti PCR
Positive
September 21, 2000
Pretransplant
Agent of HGE, IgMc
Negative
November 14, 000
Posttransplant
Agent of HGE, IgM
Negative
September 21, 2000
Pretransplant
Agent of HGE, IgG
Negative
November 14, 2000
Posttransplant
Agent of HGE, IgG
Negative
September 21, 2000
Pretransplant
Ehrlichia chaffeensis IFA
Negative
November 14, 2000
Posttransplant
E. chaffeensis IFA
Negative
September 21, 2000
Pretransplant
Lyme ELFA
Negative
November 14,2000
Posttransplant
Lyme ELFA
Negative
Implicated blood donor (tested approximately 5 months after donation)



February 28, 2001
Postdonation
Babesia microti IFA
1:256
February 28, 2001
Postdonation
B. microti PCR
Negative
February 28, 2001 Postdonation Hamster inoculationd Negative

aIFA, indirect fluorescent antibody; PCR, polymerase chain reaction; HGE, human granulocytic ehrlichiosis; Ig, immunoglobulin; ELFA, enzyme-linked fluorescence assay. 
bBabesia microti IFA≤ 1:8=negative.
cIgM and IgG immunoblots for HGE.dTwo hamsters were each inoculated intraperitoneally on April 3 with 0.75 mL of blood obtained on
February 28. Giemsa-stained smears were made from hamster blood obtained twice weekly, for 8
weeks, by tail snip. The hamsters did not become demonstrably parasitemic