Table. Results of serologic testing, polymerase chain reaction analyses, and hamster injection for specimens from the case-patient with babesiosis and the implicated blood donora.
Date of specimen | Timing of specimen | Test | Result of testing |
---|---|---|---|
Case-patient
|
|
|
|
September 21, 2000 |
Pretransplant |
Babesia microti IFA |
<1:8b |
November 14, 2000 |
Posttransplant |
B. microti IFA |
1:1024 |
November 14, 2000 |
Posttransplant |
B. microti PCR |
Positive |
September 21, 2000 |
Pretransplant |
Agent of HGE, IgMc |
Negative |
November 14, 000 |
Posttransplant |
Agent of HGE, IgM |
Negative |
September 21, 2000 |
Pretransplant |
Agent of HGE, IgG |
Negative |
November 14, 2000 |
Posttransplant |
Agent of HGE, IgG |
Negative |
September 21, 2000 |
Pretransplant |
Ehrlichia chaffeensis IFA |
Negative |
November 14, 2000 |
Posttransplant |
E. chaffeensis IFA |
Negative |
September 21, 2000 |
Pretransplant |
Lyme ELFA |
Negative |
November 14,2000 |
Posttransplant |
Lyme ELFA |
Negative |
Implicated blood donor (tested approximately 5 months after donation)
|
|
|
|
February 28, 2001 |
Postdonation |
Babesia microti IFA |
1:256 |
February 28, 2001 |
Postdonation |
B. microti PCR |
Negative |
February 28, 2001 | Postdonation | Hamster inoculationd | Negative |
aIFA, indirect fluorescent antibody; PCR, polymerase chain reaction; HGE, human granulocytic ehrlichiosis; Ig, immunoglobulin; ELFA, enzyme-linked fluorescence assay. bBabesia microti IFA≤ 1:8=negative. cIgM and IgG immunoblots for HGE. dTwo hamsters were each inoculated intraperitoneally on April 3 with 0.75 mL of blood obtained on February 28. Giemsa-stained smears were made from hamster blood obtained twice weekly, for 8 weeks, by tail snip. The hamsters did not become demonstrably parasitemic