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. Author manuscript; available in PMC: 2011 May 11.
Published in final edited form as: Biochemistry. 2010 May 11;49(18):3824–3841. doi: 10.1021/bi100055m

FIGURE 3. Dipolar ESR data for symmetric SLSs on P1, P2 and P4.

FIGURE 3

Time domain signal (left) and corresponding distance distributions (right) for P1 sites (A) A76 (B) A178 and (C) P4 A496. For P1 and P2, signals are compared in the absence (dotted line) and presence of unlabeled receptor (solid line), and for P4 in the absence (dotted line) and presence (solid line) of ATP. All time domain signals and distance distributions are scaled to a common value for ease in comparison. In (A) the concentrations of CheA, CheW and Tm14 were 25 μM, 125 μM and 225 μM, respectively.

In (B), CheA, CheW and unlabeled Tm14C concentrations were 25 μM, 125 μM and 300 μM, respectively. In (C), concentrations of spin labeled CheAΔ289, CheW and ATP were 50 μM, 100 μM and 500 μM, respectively. MgCl2 was added at (500 μM).