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. Author manuscript; available in PMC: 2011 May 11.
Published in final edited form as: Biochemistry. 2010 May 11;49(18):3824–3841. doi: 10.1021/bi100055m

FIGURE 6. Dipolar signals and distance distributions between SL-CheA:CheW and SL-receptor.

FIGURE 6

Time domain signal (left) and corresponding distance distributions (right) for Tm14C:CheA:CheW complexes. PDS data are compared for SL-CheA:CheW in the presence of unlabeled receptor (dotted lines) to the presence of spin-labeled receptor (solid lines). (A) P3 A318 to R100 and R111: Tm14C concentrations were 300 μM and CheAΔ289/CheW were at 25 μM and 125 μM, respectively. (B) P5 A634 to R100 and R111. Tm14C concentrations were 300 μM and CheAΔ289/CheW were at 25 μM and 125 μM respectively. (C) CheW 9 to R149: CheAΔ289: spin labeled CheW were at 50 μM and 100 μM respectively, whereas R149 was at 300 μM. (D) CheW 80 to R167: CheAΔ289: spin labeled CheW was at 50 μM and 100 μM, respectively whereas R167 was at 400 μM.