Table 1.
CheA/CheW intersubunit SL- distances in the absence and presence of unlabeled receptor.
| Ravg (Å) between symmetric Cys-SL sites on CheA/CheW | ||
|---|---|---|
| Free CheA/CheW | CheA/CheW/Receptor | |
| CheW | ||
| W9 | *41, 51 (28) | 41, 51 (20) |
| W31 | 50 (22) | 49 (14) |
| W80 | 56 (6) | 56 (6) |
| W139 | 63 (19) | 67 (24) |
| P1 domain | ||
| A63 | 38 (32) | 39 (36) |
| A76 | 65 (38) | 72 (38) |
| P2 domain | ||
| A178 | 34(23) | 34 (23) |
| A208 | 39 (28) | 38 (26) |
| P3 domain | ||
| A301 | 28 (6) | 28 (6) |
| A318 | 28 (6) | 28 (6) |
| A331 | 24 (9) | 24 (9) |
| P4 domain | ||
| A371 | 50 (23) | 50 (23) |
| A387 | 50 (18) | 48 (20) |
| A401C | 50 (25) | 51(29) |
| A508 | 60 (20) | 62 (21) |
| P5 domain | ||
| A545 | 44 (23) | 40 (8) |
| A553 | 63 (25) | 58 (32) |
| A568 | 62 (22) | 63 (17) |
| A634 | 40 (15) | 38 (13) |
| A639 | 45 (13) | 45 (13) |
| A646 | 58 (14) | 61 (23) |
*
Indicates the Rmax values for bimodal distribution with almost equal population of peaks. The addition of receptor leads to increase in population of one peak which is indicated in bold. Full width at half maxima for each distance distribution is reported in parenthesis. Cysteine substitutions on P3, P4 and P5 domain were made on CheAΔ289. ESR measurements on spin labeled CheW were made in presence of wild type CheAΔ289.