Figure 2.

Expression of hSOD with an amber codon at position 33 in the presence of the ${\mathrm{tRNA}}_{\mathrm{CUA}}^{\mathrm{Leu}}∕\mathrm{Anap}\text{-}2\mathrm{C}$ pair and 0.4 mM Anap. (a) SDS-PAGE analysis by GelCode Blue straining (top) and fluorescence (bottom); Lane 1: protein marker; Lane 2: hSOD expressed in the presence of the ${\mathrm{tRNA}}_{CUA}^{Leu}∕\mathrm{Anap}\text{-}2\mathrm{C}$ pair and 0.4 mM Anap; Lane 3: hSOD expressed in the presence of the ${\mathrm{tRNA}}_{\mathrm{CUA}}^{\mathrm{Leu}}∕\mathrm{Anap}\text{-}2\mathrm{C}$ pair, but in the absence of Anap. (b) ESI-MS analysis. The insert shows the deconvoluted spectum, calculated 16738, observed 16736.