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. Author manuscript; available in PMC: 2010 Sep 29.
Published in final edited form as: J Neuroimmunol. 2009 Aug 6;214(1-2):67–77. doi: 10.1016/j.jneuroim.2009.06.023

Figure 1.

Figure 1

Figure 1

Figure 1

Figure 1

GPR30 expression by immunohistochemical staining (brown) in primary immune cells and cell lines. (A) Human macrophages, (B) Mouse RAW 264.7 cells, (C) Rat microglia and (D) Human regulatory T cells all stain with an antibody against GPR30. Staining is primarily cytoplasmic in the mouse and rat cells, while in the human macrophages and T cells it is both cytoplasmic and nuclear. There is little to no staining with a control antibody. In addition, staining is abolished by preincubation with a C-terminal peptide to GPR30 but not with a scrambled version of the same peptide (D). Nuclei are lightly counterstained with hematoxylin. All images taken with a 63- oil objective on a Zeiss Axioskop. Scale bars = 100 μm in D.