GPR30 agonist treatment inhibits inflammatory cytokine expression. Splenocytes from the individual mice analyzed in Figure 6 were re-stimulated with 50 μg/ml PLP139-151 peptide in vitro. Culture supernatants were harvested after 48 h and assessed for the presence of cytokines using Beadlyte Mouse Multi-cytokine Flex Kit assay. The data are shown as mean cytokine production (pg/ml from three individual mice ±SD) in cultures from G-1-treated mice compared to cells from control-treated mice. The results indicate significant (*, p<0.05) reductions in IFN-γ, TNF, IL-12, IL-17, CCL4, and CCL5 expression. The data are representative of two similar, independent experimental replicates.