Skip to main content
. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Int J Radiat Oncol Biol Phys. 2010 Feb 1;76(2):557–565. doi: 10.1016/j.ijrobp.2009.09.024

Fig. 6. GSK-3β inhibitors prevent radiation-induced changes in pro-apoptotic proteins Bax and caspase-3 and activate anti-apoptotic protein Bcl-2.

Fig. 6

A. IEC-6 cells were pretreated with DMSO, 10 μM SB216763 or 25 μM SB415286 for 16 h, then irradiated with 6 Gy and harvested 6 h later. Cellular proteins were immunoblotted using antibodies to β-catenin, Bcl-2, Bax, and Caspase-3. Actin was used to evaluate protein loading in each lane. Relative protein levels were determined by densitometry, normalized to actin and calculated as the ratio of treated samples to sham-irradiated DMSO-pretreated controls. B. Ten week-old C57/BL6 mice were treated with daily IP injections of SB216763 (0.6 mg/kg), SB415286 (1.0 mg/kg) or DMSO for 2 days followed by 8 Gy irradiation. Four h later, proximal jejunum was fixed, sectioned and stained using antibodies to Bax and Bcl-2. Shown are representative micrographs.