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Immunoprecipitation assay to detect the oxidative modification of the tight junction protein Occludin. Total brain homogenates were immunoprecipitated with Occludin antibody, separated by SDS-PAGE and immunoblotted with anti rabbit HNE and 3NT antibodies as mentioned in the Materials and Methods section. IgG was used as a negative control, and Occludin served as the positive control. Data shown are representative of three independent experiments.
